Lattice Light Sheet image of a mouse hippocampal neuron expressing a tagged glutamate receptor (pink) concentrated at synaptic spines. Green is a GFP cell fill.
Human brain hippocampus slice. The hippocampus is the most vulnerable part of the brain in Alzheimer's disease (AD). Complexin 1 is marked in green. The synaptogyrin 1 is marked in red. These are two presynaptic proteins whose expression is decreased in AD.
"The Paint Pipette" Single-cell electroporation of ~30 pyramidal neurons with the fluorescent protein mScarlet revealing the laminar organization of the entire CA1 region of the hippocampus.
Single molecule localization based super-resolution picture of the microtubule cytoskeleton within a cell spread onto a coverslip (left corner: diffraction limited image – right: depth color coded super-resolved image). Such imaging methods allow for the acquisition of image with resolution down to 10 nm radially and 40 nm axially.
3D reconstruction of a cyst formed by stem cells grown within an alginate capsule by the startup TreFrog Therapeutics. The image was acquired using the light sheet microscopy technology called soSPIM, developed within the team Quantitative Imaging of the Cell. The color represents the depth of observation.
Single molecule localization based 3D super-resolution picture of the whole nucleus of a suspended T-cell acquired using the soSPIM imaging technique developed within the team Quantitative Imaging of the Cell. The color codes for the depth of individual molecules.
Maximum intensity projection of a fluorescently labelled mouse neuron in culture imaged by confocal microscopy, depicting the expression of neuromodulator receptors. This image also describes the concept of arborization, such as the ability of neurons to branch out in a tree-like manner, with synaptic spines resembling leaves and dendrites looking like branches.
Hippocampal neuronal in culture expressing Nlg1 (gold) and a postsynaptic marker (white)- dSTORM microscopy (top). The same image was treated with glare edges filter (bottom).
Confocal image of neurospheres from rat hippocampal cells immunostained for neuronal dendrites (MAP2, green), astrocytes (GFAP, red), cell membranes (Lamin, white) and nuclei (DAPI, blue).
Pictured is a reconstructed image of single-molecule super-resolution microscopy (dSTORM) of the subunit GluN2B of NMDA receptors, using the SR-Tesseler software.
This image shows the simulated trajectories of membrane receptors which alternate between periods of fast free diffusion in the dentritic shaft (red tracks) and confined motion at post-synaptic densities (blue tracks). FluoSim calculates in real time the positions of hundreds of individual molecules populating the neuronal geometry and provides offline display.
Purkinje neurons are the main neurons of the cerebellar cortex. They have a very complex and characteristic morphology, their intricate dendrite tree, that is flat, can be observed during whole-cell patch clamp recordings if we add to the internal solution a fluorescent molecule, such as Lucifer Yellow.
Super-resolution (STED) image of cultured hippocampal neurons filled with a fluorescent marker and showing the diversity of spine shape along dendritic shafts.
Two sides of the same coin. The same protein visualized by electrophysiology and fluorescence microscopy in cell lines and hippocampal neurons, respectively
This image represents VTA Dopamine axonal projections to the striatum acquired using STED microscopy. In this picture, we can distinguish multiple prototypical dopaminergic varicosities formed in the axonal fibers. The pseudocolor Look-Up Table "mpl-viridis" outlines the variation of signal intensity.
GFP-labeled (green) brain synapses were purified in a cell sorter, collected on glass coverslips, immunostained (magenta) and pictured at the laser scanning microscope. Semi-automated analysis extracts and organise synapses into matrices.
HCS-dSTORM on p96 of PD-1 membrane receptors of Jurkat cells, a promising target for immunotherapies. Images performed using the HCS-SMLM Imaging platform which combined localization-based super-resolution microscopy with High Content Screening approach, developed in the team Quantitative Imaging of the Cell.
Super Resolution Shadow Imaging (SUSHI) of a dentate gyrus area in a mouse hippocampal organotypic slice. By labeling the extracellular space, all cells appear as “negative” imprints, revealing an unbiased nano-anatomy of the tissue.
2-Photon image of the mouse hippocampe CA1 region. GABAergic interneurons are labelled in green, glutamatergic pyramidal cells in red. The interplay between these classes of cells is crucial for neuronal networks physiology and brain computation.
Confocal image of a hippocampal neuron in culture immunolabelled against PSD-95 (original image on the left side and inverted look-up table image on the right side).
The brain kit - This image represents components of the brain acquired by super-resolution microscopy and designed to look like the parts of a plastic toy. It reflects the spirit of bottom-up research where we study the different parts and try to put them together to understand the brain.