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Synaptic Circuits of Memory

Team Leader : Christophe Mulle

Christophe Mulle is a cellular neurobiologist with expertise in cellular electrophysiology of synaptic transmission and plasticity, receptor cell biology, generation of transgenic mice.
Since 1995, he has established a CNRS laboratory in Bordeaux which is interested in the cellular biology and pathophysiology of glutamatergic synaptic transmission and plasticity.

General objective

The research carried out in the group ambitions to link cell biological mechanisms of protein trafficking to synaptic function and dysfunction.
Great efforts are made to implement these questions at an integrated ex vivo or in vivo level in the mouse.

The general long–term objectives of the project aim at answering the following questions:

- What are the molecular mechanisms governing synapse specification and subcellular segregation of glutamate receptors in a given neuron?
- How does synaptic morphology impact on synaptic function at individual synapses, especially during developmental maturation?
- How do presynaptic and postsynaptic parameters integrate to determine proper network function?
- How does synaptic plasticity modify information transfer, network activity and contribute to memory?
- How does synaptic dysfunction cause cognitive disorders, such as mental retardation and Alzheimer’s disease?

Our projects revolve around two main scientific centers of interest: 1) kainate receptors (KARs) and their involvement in brain function and dysfunction and 2) CA3 pyramidal cells which display clearly segregated glutamatergic inputs and are a major stage of hippocampal information processing in learning and memory processes.
The studies rely on a combination of approaches ranging from molecular biology and gene-transfer in the brain to synaptic electrophysiology and live imaging. We have invested considerable effort in the development of gene-transfer methods in slices and in vivo:
(i) biolistic transfection of hippocampal slices for molecular rescue experiments, and
(ii) production of lentiviruses and AAV constructs, and in vivo sterotaxic infection in pups and young mice. These methods are fundamental for a number of projects proposed. Confocal imaging setups coupled to electrophysiology serve to couple electrophysiology to glutamate uncaging, Ca2+ imaging and morphological analysis, and Image acquisition and analysis is made possible thanks to the central imaging facility (BIC).

We are currently developing the use of electrophysiological recordings in vivo (patch-clamp and extracellular recordings), that will be ultimately combined with optogenetic stimulation of neurons.

Expertise

  • Electrophysiology of Synaptic Circuits of Memory
  • News

    Presynaptic failure in Alzheimer’s disease

    Synaptic loss is the best correlate of cognitive deficits in Alzheimer’s disease (AD). Extensive experimental evidence also indicates alterations of synaptic properties at the early stages of disease progression, before synapse loss and neuronal degeneration. A majority of studies in mouse models of AD have focused on post-synaptic mechanisms, including impairment of long-term plasticity, spine structure and glutamate receptor-mediated transmission. Here we review the literature indicating that the synaptic pathology in AD includes a strong presynaptic component. We describe the evidence indicating presynaptic physiological functions of the major molecular players in AD. These include the amyloid precursor protein (APP) and the two presenilin (PS) paralogs PS1 or PS2, genetically linked to the early-onset form of AD, in addition to tau which accumulates in a pathological form in the AD brain. Three main mechanisms participating in presynaptic functions are highlighted. APP fragments bind to presynaptic receptors (e.g. nAChRs and GABAB receptors), presenilins control Ca2+ homeostasis and Ca2+-sensors, and tau regulates the localization of presynaptic molecules and synaptic vesicles. We then discuss how impairment of these presynaptic physiological functions can explain or forecast the hallmarks of synaptic impairment and associated dysfunction of neuronal circuits in AD. Beyond the physiological roles of the AD-related proteins, studies in AD brains also support preferential presynaptic alteration. This review features presynaptic failure as a strong component of pathological mechanisms in AD.


    - Progress in Neurobiology 2020 - doi:10.1016/j.pneurobio.2020.101801
    - Authors and contacts: Gaël Barthet and Christophe Mulle

    + Cf Bordeaux Neurocampus website here

    Members

    « Researcher »

    AMEDEE Thierry Researcher thierry.amedee@u-bordeaux.fr +33533514757
    CARTA Mario Researcher mario.carta@u-bordeaux.fr +33533514759
    MULLE Christophe Researcher christophe.mulle@u-bordeaux.fr +33533514716
    POUVREAU Sandrine Researcher sandrine.pouvreau@u-bordeaux.fr +33533514760

    « Technical Staff »

    DEFORGES Severine Technical staff severine.deforges@u-bordeaux.fr +33533514780
    ERHARDT Anaël Technical staff anael.erhardt@etu.u-bordeaux.fr +33533514780
    GAUTRON Justine Technical staff justine.gautron@u-bordeaux.fr +33533514700
    GROSJEAN Noelle Technical staff noelle.grosjean@u-bordeaux.fr +33533514780
    LETENEUR Cindie Technical staff cindie.leteneur@u-bordeaux.fr +33533514700

    « Postdoc »

    BARTHET Gael Postdoc gael.barthet@u-bordeaux.fr +33533514758
    KEES Ashley Postdoc ashley.kees@u-bordeaux.fr +33533514769
    VIOTTI Julio Postdoc julio.viotti@u-bordeaux.fr +33533514700

    « PhD student »

    CUPOLILLO Dario PhD student dario.cupolillo@u-bordeaux.fr +33533514769
    GONCALVES Ania PhD student argoncalves@u-bordeaux.fr +33533514769
    JORDA SIQUIER Tomas PhD student tomas.jorda-siquier@u-bordeaux.fr +33533514769
    MARNEFFE Catherine PhD student catherine.marneffe@u-bordeaux.fr +33533514700
    MOREIRA DE SA Ana PhD student ana.moreira-de-sa@u-bordeaux.fr +33533514769

    « Student »

    REYNAUD Célia Student celia.reynaud@u-bordeaux.fr +33533514700