Membrane Trafficking at Synapses
Team Leader : David Perrais
The team (July 2020)
From left to right (without masks): Julie, Lou, Etienne,
David, Silvia, Vincent, May, Zehra, Mathias
Vesicular trafficking is one of the most salient features of synaptic physiology. In the tiny (less than 1 µm wide) chemical synapses, presynaptic vesicles concentrate and release neurotransmitter molecules which bind to post-synaptic receptors. The exocytosis and recycling of synaptic vesicles is a very prominent and essential feature of neuronal physiology that is highly controlled in time and space. Moreover, post-synaptic membrane trafficking, although not as prominent quantitatively, is pivotal for the maintenance of signal transduction complexes supporting synaptic transmission and plasticity. Most of our knowledge about synapse physiology comes from studying glutamatergic synapses which represent the majority of synapses in the brain. Nevertheless, other types of synapses, such as neuromodulatory dopaminergic synapses, could have a very different molecular composition and operate in a different way. However, because they represent a small minority of synapses formed from a very small number of neurons, their analysis has been difficult through classical cellular and molecular methods.
Our goal in the team is to use the most advanced fluorescence imaging techniques together with refined purification of synaptic elements (synaptosomes) to address the mechanisms regulating synapse function through membrane trafficking events in normal brain physiology or in the course of disease. To achieve this goal, we use, on top of the standard techniques of the modern neuroscience lab (molecular biology, biochemistry, imaging, electrophysiology), two unique expertise developed by the two PIs: first, with David Perrais, we develop methods to detect individual exocytosis and endocytosis events with pH sensitive fluorophores and perform quantitative imaging. Second, with Etienne Herzog, we purify synaptosomes from adult animals with fluorescence activated synaptosome sorting (FASS), which enables powerful proteomics, transcriptomics and functional approaches.
Altogether, we aim at identifying new pathways in specific synapses and test their relevance for synaptic nanostructure and function in the normal and diseased brain.
MemTraS: A new team at IINS
"MemTraS - Membrane traffic at synapses" is a new team recently born at IINS. The leader, David Perrais, presents the research axis:
Our goal in the team is to study the mechanisms of synapse function. We focus on membrane trafficking events, exocytosis and endocytosis, in normal brain physiology or in the course of disease. Indeed, membrane trafficking is essential in both sides of the synapse. The presynaptic element is filled with synaptic vesicles which fuse at the active zone to release neurotransmitter molecules, one of the defining features of synaptic transmission. After fusion, vesicles are very quickly and efficiently recycled to cope with neuronal activity. At the post-synaptic side, post-synaptic receptors are going through cycles of endocytosis and recycling, which is essential to regulate synaptic transmission and plasticity. We want to analyse how these processes are organized in space and time. Finally, we are not only interested in canonical synapses, such as cortical glutamatergic synapses, but also in rare and much less understood synapse populations such as neuromodulatory dopamine synapses.
To tackle these issues, we combine two types of expertise mastered by the researchers of the team, myself and Etienne Herzog. I bring electrophysiology combined with the most advanced fluorescence imaging techniques to detect and characterize individual exocytosis and endocytosis events in living cells, while Etienne brings his method of purification of synaptosomes from adult animals with fluorescence activated synaptosome sorting which enables powerful proteomics, transcriptomics and functional approaches. Altogether we aim at identifying new principles of organization in specific synapses and test their relevance for synaptic function in the normal and diseased brain.
The members of the team being formed in January 2020 are Lou Bouit, Silvia Sposini, Marlene Pfeffer, Etienne Herzog, May Bakr and David Perrais.
- More details on the Bordeaux Neurocampus website here.
- Contact: David Perrais
« Technical Staff »
|ANGIBAUD Julie||Technical email@example.com||+33533514748|
|BOUIT Lou||Technical firstname.lastname@example.org||+33533514861|
« Postdoc »
« PhD student »
|BAKR May||PhD email@example.com||+33533514779|
|LAPIOS Paul||PhD firstname.lastname@example.org||+33533514779|
« Alumni & Guests »
Magalie Martineau (post-doc, 2015-2019)
Léa Claverie (PhD student, 2015-2019)
Thi Nhu Ngoc Van (post-doc, 2014-2016)
Julia Krapivkina (PhD student, 2012-2016)
Morgane Rosendale (PhD student and post-doc, 2011-2016)
Damien Jullié (PhD student, 2008-2012)
The project: the main goal of our lab is to address the mechanisms regulating synapse function through membrane trafficking events in normal brain physiology or in the course of disease. We use the most advanced imaging techniques of exo- and endocytosis together with refined purification of synaptic elements (synaptosomes).
This project, funded by the Agence Nationale de la Recherche (ANR), is an International Collaborative Research Project with the lab of Volker Haucke (FMP Berlin, Germany).
The objective is to decipher the functional nano-architecture of the synaptic terminal and to define an active zone for synaptic vesicle endocytosis.
Your profile: PhD in biophysics and imaging, cellular neuroscience or cell biology of membrane trafficking with a good publication record and a propensity to work in a collaborative environment.
We offer: a challenging and impactful project; a diverse, collaborative and international research environment; state-of-the-art infrastructure, including unique technical expertise in imaging exo-endocytosis and synapse purification; access to high-level platforms (Bordeaux Imaging Center, proteomics, FACSility) and training opportunities.
The post-doctoral salary will be funded for an initial period of 30 months.
Please send CV, motivation letter and two references to email@example.com