Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses
Benjamin Chauvineau, Adèle Drouet, Charles Ducrot, Léa Bonamy, Tiffany Cloatre, Louis Hurson, Jérôme Baufreton, Jean-Baptiste Sibarita, Olivier Thoumine
. 2026-03-19
10.64898/2026.03.19.712855

High-affinity detection of biotinylated endogenous neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse
Charles Ducrot, Adèle Drouet, Béatrice Tessier, Chloé Desquines, Tiffany Cloâtre, Rania-Cérine Mazouzi, Florian Levet, Alexandre Favereaux, Mathieu Letellier, Olivier Thoumine
Proc. Natl. Acad. Sci. U.S.A.. 2025-05-27
10.1073/pnas.2411669122

Synaptogenic Assays Using Primary Neurons Cultured on Micropatterned Substrates
Katalin Czöndör, Nathalie Piette, Béatrice Tessier, Vincent Studer, Olivier Thoumine
Methods in Molecular Biology. 2025-01-01
10.1007/978-1-0716-4446-1_1

LRRTM2 controls presynapse nano-organization and AMPA receptor sub-positioning through Neurexin-binding interface
Konstantina Liouta, Malgorzata Lubas, Vasika Venugopal, Julia Chabbert, Caroline Jeannière, Candice Diaz, Matthieu Munier, Béatrice Tessier, Stéphane Claverol, Alexandre Favereaux, Matthieu Sainlos, Joris de Wit, Mathieu Letellier, Olivier Thoumine, Ingrid Chamma
Nat Commun. 2024-10-11
10.1038/s41467-024-53090-y

Protocol for matching protein localization to synapse morphology in primary rat neurons by correlative super-resolution microscopy
Tiffany Cloâtre, Magali Mondin, Jean-Baptiste Sibarita, Florian Levet, Olivier Thoumine
STAR Protocols. 2024-09-01
10.1016/j.xpro.2024.103160

Neuroligin-1 dependent phosphotyrosine signaling in excitatory synapse differentiation
Zsófia Szíber, Adèle Drouet, Magali Mondin, Florian Levet, Olivier Thoumine
Front. Mol. Neurosci.. 2024-05-15
10.3389/fnmol.2024.1359067

miR ‐124‐dependent tagging of synapses by synaptopodin enables input‐specific homeostatic plasticity
Sandra Dubes, Anaïs Soula, Sébastien Benquet, Béatrice Tessier, Christel Poujol, Alexandre Favereaux, Olivier Thoumine, Mathieu Letellier
The EMBO Journal. 2022-07-25
10.15252/embj.2021109012

MDGAs are fast-diffusing molecules that delay excitatory synapse development by altering neuroligin behavior.
Andrea Toledo, Mathieu Letellier, Giorgia Bimbi, Béatrice Tessier, Sophie Daburon, Alexandre Favereaux, Ingrid Chamma, Kristel Vennekens, Jeroen Vanderlinden, Matthieu Sainlos, Joris de Wit, Daniel Choquet, Olivier Thoumine
eLife. 2022-05-09
10.7554/elife.75233

High-Resolution Fluorescence Imaging Combined With Computer Simulations to Quantitate Surface Dynamics and Nanoscale Organization of Neuroligin-1 at Synapses.
Matthieu Lagardère, Adèle Drouet, Matthieu Sainlos, Olivier Thoumine
Front. Synaptic Neurosci.. 2022-04-25
10.3389/fnsyn.2022.835427

Convergence of adenosine and GABA signaling for synapse stabilization during development.
Ferran Gomez-Castro, Stefania Zappettini, Jessica C. Pressey, Carla G. Silva, Marion Russeau, Nicolas Gervasi, Marta Figueiredo, Claire Montmasson, Marianne Renner, Paula M. Canas, Francisco Q. Gonçalves, Sofia Alçada-Morais, Eszter Szabó, Ricardo J. Rodrigues, Paula Agostinho, Angelo R. Tomé, Ghislaine Caillol, Olivier Thoumine, Xavier Nicol, Christophe Leterrier, Rafael Lujan, Shiva K. Tyagarajan, Rodrigo A. Cunha, Monique Esclapez, Christophe Bernard, Sabine Lévi
Science. 2021-11-05
10.1126/science.abk2055

Role of regulatory C‐terminal motifs in synaptic confinement of LRRTM2
Konstantina Liouta, Julia Chabbert, Sebastien Benquet, Béatrice Tessier, Vincent Studer, Matthieu Sainlos, Joris De Wit, Olivier Thoumine, Ingrid Chamma
Biol. Cell. 2021-09-27
10.1111/boc.202100026

SlitC-PlexinA1 mediates iterative inhibition for orderly passage of spinal commissural axons through the floor plate.
Hugo Ducuing, Thibault Gardette, Aurora Pignata, Karine Kindbeiter, Muriel Bozon, Olivier Thoumine, Céline Delloye-Bourgeois, Servane Tauszig-Delamasure, Valerie Castellani
eLife. 2020-12-21
10.7554/elife.63205

FluoSim: simulator of single molecule dynamics for fluorescence live-cell and super-resolution imaging of membrane proteins.
Matthieu Lagardère, Ingrid Chamma, Emmanuel Bouilhol, Macha Nikolski, Olivier Thoumine
Sci Rep. 2020-11-17
10.1038/s41598-020-75814-y

Alternative splicing at neuroligin site A regulates glycan interaction and synaptogenic activity
Shinichiro Oku, Huijuan Feng, Steven Connor, Andrea Toledo, Peng Zhang, Yue Zhang, Olivier Thoumine, Chaolin Zhang, Ann Marie Craig
eLife. 2020-09-11
10.7554/elife.58668

Optogenetic control of excitatory post-synaptic differentiation through neuroligin-1 tyrosine phosphorylation
Mathieu Letellier, Matthieu Lagardère, Béatrice Tessier, Harald Janovjak, Olivier Thoumine
eLife. 2020-04-23
10.7554/elife.52027

FluoSim: simulator of single molecule dynamics for fluorescence live-cell and super-resolution imaging of membrane proteins
Matthieu Lagardère, Ingrid Chamma, Emmanuel Bouilhol, Macha Nikolski, Olivier Thoumine
. 2020-02-06
10.1101/2020.02.06.937045

Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth
Steve Dos-Santos Carvalho, Maite M Moreau, Yeri Esther Hien, Michael Garcia, Nathalie Aubailly, Deborah J Henderson, Vincent Studer, Nathalie Sans, Olivier Thoumine, Mireille Montcouquiol
eLife. 2020-01-07
10.7554/eLife.51822

miRNA-Dependent Control of Homeostatic Plasticity in Neurons
Sandra Dubes, Alexandre Favereaux, Olivier Thoumine, Mathieu Letellier
Front. Cell. Neurosci.. 2019-12-05
10.3389/fncel.2019.00536

A super-resolution platform for correlative live single-molecule imaging and STED microscopy
V. V. G. Krishna Inavalli, Martin O. Lenz, Corey Butler, Julie Angibaud, Benjamin Compans, Florian Levet, Jan Tønnesen, Olivier Rossier, Gregory Giannone, Olivier Thoumine, Eric Hosy, Daniel Choquet, Jean-Baptiste Sibarita, U. Valentin Nägerl
Nat Methods. 2019-10-21
10.1038/s41592-019-0611-8

TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering
Edoardo Moretto, Anna Longatti, Luca Murru, Ingrid Chamma, Alessandro Sessa, Jonathan Zapata, Eric Hosy, Matthieu Sainlos, Julien Saint-Pol, Eric Rubinstein, Daniel Choquet, Vania Broccoli, Giampietro Schiavo, Olivier Thoumine, Maria Passafaro
Cell Reports. 2019-10-01
10.1016/j.celrep.2019.09.051

A Spatiotemporal Sequence of Sensitization to Slits and Semaphorins Orchestrates Commissural Axon Navigation.
Aurora Pignata, Hugo Ducuing, Leila Boubakar, Thibault Gardette, Karine Kindbeiter, Muriel Bozon, Servane Tauszig-Delamasure, Julien Falk, Olivier Thoumine, Valérie Castellani
Cell Reports. 2019-10-01
10.1016/j.celrep.2019.08.098

Differential role of pre- and postsynaptic neurons in the activity-dependent control of synaptic strengths across dendrites
Mathieu Letellier, Florian Levet, Olivier Thoumine, Yukiko Goda
PLoS Biol. 2019-06-05
10.1371/journal.pbio.2006223

Biophysical mechanisms underlying the membrane trafficking of synaptic adhesion molecules.
Ingrid Chamma, Matthieu Sainlos, Olivier Thoumine
Neuropharmacology. 2019-03-01
10.1016/j.neuropharm.2019.02.037

A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation
Mathieu Letellier, Zsófia Szíber, Ingrid Chamma, Camille Saphy, Ioanna Papasideri, Béatrice Tessier, Matthieu Sainlos, Katalin Czöndör, Olivier Thoumine
Nat Commun. 2018-09-28
10.1038/s41467-018-06220-2

Dynamics, nanoscale organization, and function of synaptic adhesion molecules
Ingrid Chamma, Olivier Thoumine
Molecular and Cellular Neuroscience. 2018-09-01
10.1016/j.mcn.2018.04.007

Pre-post synaptic alignment through neuroligin-1 tunes synaptic transmission efficiency
Kalina T Haas, Benjamin Compans, Mathieu Letellier, Thomas M Bartol, Dolors Grillo-Bosch, Terrence J Sejnowski, Matthieu Sainlos, Daniel Choquet, Olivier Thoumine, Eric Hosy
eLife. 2018-07-25
10.7554/eLife.31755

A Triad of Crystals Sheds Light on MDGA Interference with Neuroligation.
Olivier Thoumine, Pascale Marchot
Neuron. 2017-08-01
10.1016/j.neuron.2017.08.001

Defective Gpsm2/Gαi3 signalling disrupts stereocilia development and growth cone actin dynamics in Chudley-McCullough syndrome.
Stephanie A. Mauriac, Yeri E. Hien, Jonathan E. Bird, Steve Dos-Santos Carvalho, Ronan Peyroutou, Sze Chim Lee, Maite M. Moreau, Jean-Michel Blanc, Aysegul Geyser, Chantal Medina, Olivier Thoumine, Sandra Beer-Hammer, Thomas B. Friedman, Lukas Rüttiger, Andrew Forge, Bernd Nürnberg, Nathalie Sans, Mireille Montcouquiol
Nat Comms. 2017-04-07
10.1038/ncomms14907

Optimized labeling of membrane proteins for applications to super-resolution imaging in confined cellular environments using monomeric streptavidin
Ingrid Chamma, Olivier Rossier, Grégory Giannone, Olivier Thoumine, Matthieu Sainlos
Nat Protoc. 2017-03-09
10.1038/nprot.2017.010

Synaptogenic Assays Using Neurons Cultured on Micropatterned Substrates.
Katalin Czöndör, Olivier Thoumine
Methods in Molecular Biology. 2016-12-11
10.1007/978-1-4939-6688-2_3

Nanoscale organization of synaptic adhesion proteins revealed by single-molecule localization microscopy
Ingrid Chamma, Florian Levet, Jean-Baptiste Sibarita, Matthieu Sainlos, Olivier Thoumine
Neurophoton. 2016-11-03
10.1117/1.NPh.3.4.041810

Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells.
Sabine Fièvre, Mario Carta, Ingrid Chamma, Virginie Labrousse, Olivier Thoumine, Christophe Mulle
Nat Comms. 2016-09-27
10.1038/ncomms12738

Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin
Ingrid Chamma, Mathieu Letellier, Corey Butler, Béatrice Tessier, Kok-Hong Lim, Isabel Gauthereau, Daniel Choquet, Jean-Baptiste Sibarita, Sheldon Park, Matthieu Sainlos, Olivier Thoumine
Nature Communications. 2016-03-16
10.1038/ncomms10773

The Sorting Receptor SorCS1 Regulates Trafficking of Neurexin and AMPA Receptors
Jeffrey N. Savas, Luís F. Ribeiro, Keimpe D. Wierda, Rebecca Wright, Laura A. DeNardo-Wilke, Heather C. Rice, Ingrid Chamma, Yi-Zhi Wang, Roland Zemla, Mathieu Lavallée-Adam, Kristel M. Vennekens, Matthew L. O’Sullivan, Joseph K. Antonios, Elizabeth A. Hall, Olivier Thoumine, Alan D. Attie, John R. Yates, Anirvan Ghosh, Joris de Wit
Neuron. 2015-08-01
10.1016/j.neuron.2015.08.007

Two-tiered coupling between flowing actin and immobilized N-cadherin/catenin complexes in neuronal growth cones
Mikael Garcia, Cécile Leduc, Matthieu Lagardère, Amélie Argento, Jean-Baptiste Sibarita, Olivier Thoumine
Proc Natl Acad Sci USA. 2015-05-18
10.1073/pnas.1423455112

Mechanical coupling between transsynaptic N-cadherin adhesions and actin flow stabilizes dendritic spines.
Anaël Chazeau, Mikael Garcia, Katalin Czöndör, David Perrais, Béatrice Tessier, Grégory Giannone, Olivier Thoumine
MBoC. 2015-03-01
10.1091/mbc.E14-06-1086

Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusion.
Anaël Chazeau, Amine Mehidi, Deepak Nair, Jérémie J Gautier, Cécile Leduc, Ingrid Chamma, Frieda Kage, Adel Kechkar, Olivier Thoumine, Klemens Rottner, Daniel Choquet, Alexis Gautreau, Jean‐Baptiste Sibarita, Grégory Giannone
EMBO J.. 2014-10-07
10.15252/embj.201488837

miR-92a regulates expression of synaptic GluA1-containing AMPA receptors during homeostatic scaling.
Mathieu Letellier, Sara Elramah, Magali Mondin, Anaïs Soula, Andrew Penn, Daniel Choquet, Marc Landry, Olivier Thoumine, Alexandre Favereaux
Nat Neurosci. 2014-07-13
10.1038/nn.3762

Micropatterned substrates coated with neuronal adhesion molecules for high-content study of synapse formation.
Katalin Czöndör, Mikael Garcia, Amélie Argento, Audrey Constals, Christelle Breillat, Béatrice Tessier, Olivier Thoumine
Nat Commun. 2013-08-12
10.1038/ncomms3252

Assembly of synapses: biomimetic assays to control neurexin/neuroligin interactions at the neuronal surface.
Magali Mondin, Béatrice Tessier, Olivier Thoumine
Current Protocols in Neuroscience. 2013-07-01
10.1002/0471142301.ns0219s64

Neurexin-1β Binding to Neuroligin-1 Triggers the Preferential Recruitment of PSD-95 versus Gephyrin through Tyrosine Phosphorylation of Neuroligin-1
Grégory Giannone, Magali Mondin, Dolors Grillo-Bosch, Béatrice Tessier, Edouard Saint-Michel, Katalin Czöndör, Matthieu Sainlos, Daniel Choquet, Olivier Thoumine
Cell Reports. 2013-06-01
10.1016/j.celrep.2013.05.013

Biophysical mechanisms regulating AMPA receptor accumulation at synapses.
Katalin Czöndör, Olivier Thoumine
Brain Research Bulletin. 2013-04-01
10.1016/j.brainresbull.2012.11.001

Unified quantitative model of AMPA receptor trafficking at synapses
K. Czondor, M. Mondin, M. Garcia, M. Heine, R. Frischknecht, D. Choquet, J.-B. Sibarita, O. R. Thoumine
Proceedings of the National Academy of Sciences. 2012-02-13
10.1073/pnas.1109818109

Neurexin-neuroligin adhesions capture surface-diffusing AMPA receptors through PSD-95 scaffolds.
M. Mondin, V. Labrousse, E. Hosy, M. Heine, B. Tessier, F. Levet, C. Poujol, C. Blanchet, D. Choquet, O. Thoumine
Journal of Neuroscience. 2011-09-21
10.1523/jneurosci.6439-10.2011

Bidirectional integrative regulation of Cav1.2 calcium channel by microRNA miR-103: role in pain.
Alexandre Favereaux, Olivier Thoumine, Rabia Bouali-Benazzouz, Virginie Roques, Marie-Amélie Papon, Sherine Abdel Salam, Guillaume Drutel, Claire Léger, André Calas, Frédéric Nagy, Marc Landry
The EMBO Journal. 2011-07-29
10.1038/emboj.2011.249

Multi-level molecular clutches in motile cell processes.
Grégory Giannone, René-Marc Mège, Olivier Thoumine
Trends in Cell Biology. 2009-09-01
10.1016/j.tcb.2009.07.001

Neurexin/neuroligin interaction kinetics characterized by counting single cell-surface attached quantum dots.
Edouard Saint-Michel, Grégory Giannone, Daniel Choquet, Olivier Thoumine
Biophysical Journal. 2009-07-01
10.1016/j.bpj.2009.04.044

Activity-independent and subunit-specific recruitment of functional AMPA receptors at neurexin/neuroligin contacts.
M. Heine, O. Thoumine, M. Mondin, B. Tessier, G. Giannone, D. Choquet
Proceedings of the National Academy of Sciences. 2008-12-19
10.1073/pnas.0804007106

Interplay between adhesion turnover and cytoskeleton dynamics in the control of growth cone migration.
Olivier Thoumine
Cell Adhesion & Migration. 2008-10-01
10.4161/cam.2.4.7274

A molecular clutch between the actin flow and N-cadherin adhesions drives growth cone migration
L. Bard, C. Boscher, M. Lambert, R.-M. Mege, D. Choquet, O. Thoumine
Journal of Neuroscience. 2008-06-04
10.1523/JNEUROSCI.5331-07.2008

Probing the dynamics of protein-protein interactions at neuronal contacts by optical imaging.
Olivier Thoumine, Helge Ewers, Martin Heine, Laurent Groc, Renato Frischknecht, Grégory Giannone, Christel Poujol, Philippe Legros, Brahim Lounis, Laurent Cognet, Daniel Choquet
Chem. Rev.. 2008-05-01
10.1021/cr078204m

Nucleation and growth of cadherin adhesions
Mireille Lambert, Olivier Thoumine, Julien Brevier, Daniel Choquet, Daniel Riveline, René-Marc Mège
Experimental Cell Research. 2007-11-01
10.1016/j.yexcr.2007.07.035

Characterization of SynCAM surface trafficking using a SynCAM derived ligand with high homophilic binding affinity
Christelle Breillat, Olivier Thoumine, Daniel Choquet
Biochemical and Biophysical Research Communications. 2007-08-01
10.1016/j.bbrc.2007.05.152

Fast turnover of L1 adhesions in neuronal growth cones involving both surface diffusion and exo/endocytosis of L1 molecules
Caroline Dequidt, Lydia Danglot, Philipp Alberts, Thierry Galli, Daniel Choquet, Olivier Thoumine
MBoC. 2007-08-01
10.1091/mbc.E06-12-1101

Extracellular Interactions between GluR2 and N-Cadherin in Spine Regulation
Laura Saglietti, Caroline Dequidt, Kinga Kamieniarz, Marie-Claude Rousset, Pamela Valnegri, Olivier Thoumine, Francesca Beretta, Laurent Fagni, Daniel Choquet, Carlo Sala, Morgan Sheng, Maria Passafaro
Neuron. 2007-05-01
10.1016/j.neuron.2007.04.012

Regulation of N-cadherin dynamics at neuronal contacts by ligand binding and cytoskeletal coupling
Olivier Thoumine, Mireille Lambert, René-Marc Mège, Daniel Choquet
MBoC. 2006-02-01
10.1091/mbc.E05-04-0335

Weak effect of membrane diffusion on the rate of receptor accumulation at adhesive contacts
Olivier Thoumine, Edouard Saint-Michel, Caroline Dequidt, Julien Falk, Rachel Rudge, Thierry Galli, Catherine Faivre-Sarrailh, Daniel Choquet
Biophysical Journal. 2005-11-01
10.1529/biophysj.105.071688

NrCAM coupling to the cytoskeleton depends on multiple protein domains and partitioning into lipid rafts
Julien Falk, Olivier Thoumine, Caroline Dequidt, Daniel Choquet, Catherine Faivre-Sarrailh
MBoC. 2004-10-01
10.1091/mbc.E04-03-0171

Predicting the kinetics of cell spreading.
Thomas Frisch, Olivier Thoumine
Journal of Biomechanics. 2002-08-01
10.1016/s0021-9290(02)00075-1

Contribution of the nucleus to the mechanical properties of endothelial cells.
Nathalie Caille, Olivier Thoumine, Yanik Tardy, Jean-Jacques Meister
Journal of Biomechanics. 2002-02-01
10.1016/s0021-9290(01)00201-9

Dynamics of adhesive rupture between fibroblasts and fibronectin: microplate manipulations and deterministic model.
Olivier Thoumine, Jean-Jacques Meister
Eur Biophys J. 2000-09-22
10.1007/pl00006648

Short-term binding of fibroblasts to fibronectin: optical tweezers experiments and probabilistic analysis.
Olivier Thoumine, Pierre Kocian, Arlette Kottelat, Jean-Jacques Meister
European Biophysics Journal. 2000-09-22
10.1007/s002490000087

A probabilistic model for ligand-cytoskeleton transmembrane adhesion: predicting the behavior of microspheres on the surface of migrating cells.
OLIVIER THOUMINE, JEAN-JACQUES MEISTER
Journal of Theoretical Biology. 2000-06-01
10.1006/jtbi.2000.2024

Changes in the mechanical properties of fibroblasts during spreading: a micromanipulation study.
O. Thoumine, Olivier Cardoso, Jean-Jacques Meister
European Biophysics Journal. 1999-03-09
10.1007/s002490050203

Microplates: a new tool for manipulation and mechanical perturbation of individual cells.
Olivier Thoumine, Albrecht Ott, Olivier Cardoso, Jean-Jacques Meister
Journal of Biochemical and Biophysical Methods. 1999-02-01
10.1016/s0165-022x(98)00052-9

Time scale dependent viscoelastic and contractile regimes in fibroblasts probed by microplate manipulation.
O. Thoumine, A. Ott
Journal of Cell Science. 1997-09-01
10.1242/jcs.110.17.2109

Comparison of the mechanical properties of normal and transformed fibroblasts.
O THOUMINE, A OTT
Biorheology. 1997-07-01
10.1016/s0006-355x(98)00007-9

Critical centrifugal forces induce adhesion rupture or structural reorganization in cultured cells.
Olivier Thoumine, Albrecht Ott, Daniel Louvard
Cell Motil. Cytoskeleton. 1996-01-01
10.1002/(sici)1097-0169(1996)33:43.0.co;2-7

Influence of adhesion and cytoskeletal integrity on fibroblast traction.
Olivier Thoumine, Albrecht Ott
Cell Motil. Cytoskeleton. 1996-01-01
10.1002/(sici)1097-0169(1996)35:33.0.co;2-3

Elongation of confluent endothelial cells in culture: the importance of fields of force in the associated alterations of their cytoskeletal structure.
Olivier Thoumine, Thierry Ziegler, Peggy R. Girard, Robert M. Nerem
Experimental Cell Research. 1995-08-01
10.1006/excr.1995.1249

Oscillatory shear stress and hydrostatic pressure modulate cell-matrix attachment proteins in cultured endothelial cells.
Olivier Thoumine, Robert M. Nerem, Feggy R. Girard
In Vitro Cell Dev Biol - Animal. 1995-01-01
10.1007/bf02631337